Barcelona beat Hamburg 2-1 on goals by Dani Alves and Gerard Deulofeu. The team was also without its Spanish national team players, still on vacation after winning the European Championship final in Ukraine on July 1.
Barcelona said Messi's response to treatment will determine how long he is out.
The Spanish club plays Dinamo Bucharest in an exhibition on August 11 and the absence of Messi could cost the Catalans even more money.
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